polyclonal rabbit anti phospho mdm2 ser186  (Bioss)

Journal: bioRxiv

Article Title: Regulation of the MDM2-p53 Nexus by a Nuclear Phosphoinositide and Small Heat Shock Protein Complex

doi: 10.1101/2025.04.11.648454

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Article Snippet: Monoclonal antibodies against PIP 2 (clone 2C11, #Z-P045, Echelon Biosciences), PIP 2 (clone KT10, #MSBS2283, MilliporeSigma), p53 (clone DO-1, #SC-126, Santa Cruz Biotechnology), HSP27 (clone F-4, #SC-13132, Santa Cruz Biotechnology), αBC (D6S9E, #45844, Cell Signaling), PIPKIIα (PIP4K2A, clone D83C1, #5527, Cell Signaling), PTEN (clone D4,3, #9188, Cell Signaling), GAPDH (clone 0411, #SC-47724, Santa Cruz Biotechnology), and polyclonal antibodies against MDM2 (clone D1V2Z, #86934, Cell Signaling), MDM2 (#AF1244, R&D Systems), αBC (#ab13497, Abcam), PIPKIα (PIP5K1A, #9693, Cell Signaling), PIPKIγ (PIP5K1C, #3296, Cell Signaling), PIPKIIβ (PIP4K2B, #9694, Cell Signaling), PITPNB (#NBP2-19841, Novus Biologicals), FOXO3a (clone 75D8, #2497, Cell Signaling), Histone H2A (#2578, Cell Signaling), Histone H2B (clone D2H6, #12364, Cell Signaling), Ubiquitin (P37, #58395, Cell Signaling) were utilized in this study.

Techniques: Binding Assay, Fluorescence, Microscale Thermophoresis, Concentration Assay, Incubation, Control, Software, Bacteria

Journal: eLife

Article Title: Mechanically stimulated osteocytes maintain tumor dormancy in bone metastasis of non-small cell lung cancer by releasing small extracellular vesicles

doi: 10.7554/eLife.89613

Figure Lengend Snippet: ( a ) Comparison of relative miR-365-1-5p, miR-99b-3p, miR-193a-5p, miR-125a-3p, miR-690, miR-1195, miR-122-5p, miR-140-3p content between MLO-Y4-sEVs and MC3T3-E1-sEVs by real-time quantitative polymerase chain reaction (qRT-PCR). n=3, Student’s two-sided unpaired t test. ( b ) NSCLC cells were transfected with miR-365a-5p, miR-30c-1-3p, miR-193a-5p, miR-99b-3p mimics, or NC (negative control) in Dulbecco’s modified eagle medium (DMEM). 48 hr later, Cell Counting Kit-8 (CCK-8) assays were performed. n=6, one-way analysis of variance with Turkey’s multiple comparisons test. ( c, d ) NSCLC cells were transfected with miR-99b-3p mimics, NC mimics, miR-99b-3p inhibitor, and NC inhibitor. 48 hr later, EdU flow cytometry were performed. CCK-8 assays were performed at different points in time after transfection. n=6, two-way analysis of variance with Sidak’s multiple comparisons test. ( e ) Schematic diagram of putative miR-99b-3p binding sites in the MDM2 3′-UTR. Green letters denote mutation sites. Relative luciferase activities of wild-type (WT) or mutant (MUT) MDM2 3′-UTRs were determined in A549 cells, which were co-transfected with the miR-99b-3p mimics or negative control. Luciferase activity was normalized using Renilla. n=4, Student’s two-sided unpaired t test. ( f ) MDM2 protein in A549 cells was analyzed by western blot 72 hr after transfection. n=3; one-way analysis of variance with Turkey’s multiple comparisons test. Error bars represent Mean ± SD. No significance (ns) p>0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Figure 3—source data 1. Original table sources for quantification of plots. Figure 3—source data 2. Original western blot images for . Figure 3—source data 3. Original western blot images for with highlighted bands and sample labels.

Article Snippet: Antibody , MDM2 (Rabbit polyclonal) , Proteintech , 66511-1-IG , 1:1000.

Techniques: Comparison, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Modification, Cell Counting, CCK-8 Assay, Flow Cytometry, Binding Assay, Mutagenesis, Luciferase, Activity Assay, Western Blot

Journal: eLife

Article Title: Mechanically stimulated osteocytes maintain tumor dormancy in bone metastasis of non-small cell lung cancer by releasing small extracellular vesicles

doi: 10.7554/eLife.89613

Figure Lengend Snippet: Osteocytes, sensing mechanical stimulation generated by exercise or mechanical loading, inhibit the proliferation and sustain the dormancy of NSCLC cells by releasing small extracellular vesicles (sEVs) with tumor suppressor micro-RNAs (miRNAs), such as miR-99b-3p, which inhibits the proliferation of NSCLC cells by directly targeting murine double minute 2 (MDM2).

Article Snippet: Antibody , MDM2 (Rabbit polyclonal) , Proteintech , 66511-1-IG , 1:1000.

Techniques: Generated

Journal: eLife

Article Title: Mechanically stimulated osteocytes maintain tumor dormancy in bone metastasis of non-small cell lung cancer by releasing small extracellular vesicles

doi: 10.7554/eLife.89613

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Article Snippet: Antibody , MDM2 (Rabbit polyclonal) , Proteintech , 66511-1-IG , 1:1000.

Techniques: Sequencing, In Vitro, Software